Florida isolate 3 - SRA

SRX1392519: Florida isolate 3
1 ILLUMINA (Illumina HiSeq 2500) run: 3.9M spots, 539.8M bases, 426.2Mb downloads

Design: Genomic DNA from each individual was digested with Sbf I-HF restriction enzyme was ligated to the unique P1 RAD adapter primers. Adapter ligated DNA fragments from individual samples were pooled and sonicated. DNA fragments between 400-600 bp were extracted using 1% agarose gel. Blunt ends were repaired and A-overhangs were then added using Klenow fragment (3’-5’ exo). P2 RAD adapter was then ligated to the DNA.

Submitted by: Purdue University

Study: Ixodes scapularis genome sequencing

PRJNA299499 • SRP065406 • All experiments • All runs

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Illumina RADseq project to evaluate the genetic structure and population genomics of the black-legged tick, Ixodes scapularis (the tick vector of Lyme disease, babesiosis, human granulocytic anaplasmosis and Powassan virus) across North America.

Sample: Ixodes scapularis Florida Female 3

SAMN04215328 • SRS1137737 • All experiments • All runs

Organism: Ixodes scapularis

Library:

Name: FL3

Instrument: Illumina HiSeq 2500

Strategy: WGS

Source: GENOMIC

Selection: Restriction Digest

Layout: SINGLE

Spot descriptor:

1 forward

Runs: 1 run, 3.9M spots, 539.8M bases, 426.2Mb

Run	# of Spots	# of Bases	Size	Published
SRR2845394	3,855,569	539.8M	426.2Mb	2015-12-15

ID:: 1978278

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